Journal: Virus research

Article Title: The therapeutic effect of death: Newcastle disease virus and its antitumor potential

doi: 10.1016/j.virusres.2015.07.001

Figure Lengend Snippet: Confocal microscopy images of HeLa cells infected with the recombinant rNDV-B1/Fas virus (Cuadrado-Castano et al., 2015). Left: composite Z-stack of six optical slices showing Hela-infected cells undergoing apoptosis; highlighted are different stages of the apoptosis response observed among the cell population identified accordingly to the morphological changes observed during the progression of apoptosis cell death: pre-apoptosis stage (lack of morphological changes); active apoptosis (A) showing cellular shrinkage and DNA fragmentation. Advance apoptosis (B) displaying extensive membrane blebbing and DNA fragmentation. Late apoptosis (C) showing apoptotic bodies scale bar 100 µm. Right plots: single stacks and magnification of morphological features, observed in the main left panel: DNA fragmentation (A), membrane blebbing (B) or C (apoptotic bodies). Scale bar 10 µm. For the propose of this image, the cells were infected at an MOI of 1 PFU/cell, fixed 20 h after infection, and stained with monoclonal anti-human Fas antibody (red), polyclonal anti-NDV serum (green) and Hoechst for nuclear contrast. Confocal laser scanning was performed using a Zeiss LSM 190 510 Meta (Carl Zeiss Microimaging, Thornwood, NY) fitted with a Plan Apochromatic 63×/1.4 191. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).

Article Snippet: Confocal laser scanning was performed using a Zeiss LSM 190 510 Meta (Carl Zeiss Microimaging, Thornwood, NY) fitted with a Plan Apochromatic 63×/1.4 191. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).

Techniques: Confocal Microscopy, Infection, Recombinant, Staining